Humanized antibody mainly refers to the re-expressed mouse monoclonal antibody modified by gene cloning and DNA recombination technology. Most of its amino acid sequence is replaced by human sequence, which basically retains the affinity and specificity of the parent mouse monoclonal antibody, but reduces Because of its heterogeneity, it can be applied to the human body. Humanized antibody means that the constant region of the antibody (ie, CH and CL regions) or all of the antibody is encoded by human antibody genes. Humanized antibodies can greatly reduce the immune side effects caused by heterologous antibodies to the human body. Humanized antibodies include chimeric antibodies, modified antibodies and fully humanized antibodies.
Figure 1. Antibody humanization.
The current general computational antibody design method starts with the modeling of antibodies and antibody-antigen complexes. We select antibody sequences for antigen binding experiments, for example, bind to soluble antigens or antigens expressed by cells, and further optimize conjugates through affinity maturation methods. However, this method usually requires modeling the entire structure, which requires a lot of calculations and takes a long time. Therefore, MedAI proposes an antibody design based on the CDR of the specific binding site of the antibody and the antigen. This method simplifies the calculation and design of antibodies, simplifies the modeling and design of antibody CDRs, and greatly reduces the calculation requirements.
MedAI uses computer-aided structural simulation design to humanize mouse monoclonal antibodies and rabbit monoclonal antibodies.
Antibody sequence annotation: annotation of antibody heavy and light chain variable regions, constant regions and CDR regions, recognition of antibody germline genes.
Antibody sequence analysis: predict antibody post-translational modification sites (such as glycosylation sites, etc.) and predict antigen linear epitopes.
Antibody structure prediction and optimization: predict the structure of full-length antibodies, antibody Fab regions, and scFv.
Humanized antibody design, immunogenicity prediction.
Antibody-antigen interaction prediction: Identify the conformational epitope of the antigen and determine the hapten connection site.
Design and modification of antibody molecules: antibody affinity maturation, antibody stability optimization, bispecific antibody design, antibody aggregation effect analysis.
|Project name||Antibody Humanization Service|
|Cycle||Depends on the time you need to simulate and the time required for the system to reach equilibrium.|
|Product delivery mode||The simulation results provide you with the raw data and analysis results of molecular dynamics.|
MedAI's antibody humanization service can reduce the cost of subsequent experiments. Antibody humanization service is a personalized and customized innovative scientific research service. Before determining the corresponding analysis plan and price, each project needs to be evaluated. If you want to know more about service prices or technical details, please feel free to contact us. If you want to know more about service prices or technical details, please feel free to contact us.
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